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Updated: Feb 5, 2026

Ex Vivo Organotypic Corneal Model of Acute Epithelial Herpes Simplex Virus Type I Infection
Published on: November 3, 2012
Liquid Nitrogen Cryotherapy Treatment of Herpes Simplex Epithelial Keratitis
Frederick W Fraunfelder1, Shashi Alloju2, Michelle Patel3
1Frederick W. Fraunfelder, MD, MSMA member since 2014, is chairman and the Roy E. Mason and Elizabeth Patee Mason Distinguished Professor of the Department of Ophthalmology, Mason Eye Institute, at the University of Missouri.
Abstract:
A retrospective case series was performed in a university setting for all patients with herpes simplex epithelial keratitis who underwent liquid nitrogen cryotherapy from 2012-2015. Outcome measure was clinical evidence of resolution of epithelial keratitis in the cornea with re-epithelialization. All cases of epithelial keratitis showed partial and complete resolution at day 1 and week 1 respectively. Liquid nitrogen cryotherapy is a safe and effective treatment for herpes epithelial keratitis comparable to other published studies.
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