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Related Experiment Videos

SV40 large T-antigen: dual oncogene.

J S Butel

    Cancer Surveys
    |January 1, 1986
    PubMed
    Summary

    Simian virus 40 (SV40) large tumor antigen (T-ag) acts as a dual oncogene, with nuclear and membrane forms driving cell transformation. These distinct T-ag functions cooperate to alter cell morphology and immortalize cells.

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    Area of Science:

    • Oncology
    • Virology
    • Molecular Biology

    Background:

    • Simian virus 40 (SV40) is a DNA tumor virus.
    • SV40 large tumor antigen (T-ag) is crucial for viral replication and cell transformation.
    • T-ag exhibits distinct functions at different subcellular locations.

    Purpose of the Study:

    • To investigate the dual oncogenic functions of SV40 T-ag.
    • To elucidate the roles of nuclear and membrane-localized T-ag in cell transformation.
    • To understand the interaction between T-ag and cellular protein p53.

    Main Methods:

    • Analysis of T-ag's molecular weight and functional domains.
    • Investigation of T-ag localization (nucleus and plasma membrane).
    • Examination of T-ag post-translational modifications (acylation).

    Main Results:

    • T-ag complexes with and stabilizes cellular p53.
    • Nuclear T-ag is essential for cell immortalization.
    • Membrane T-ag contributes to significant morphological changes.
    • Nuclear and membrane T-ag forms are structurally similar but differentially modified.

    Conclusions:

    • SV40 T-ag functions as a dual oncogene with distinct nuclear and membrane-associated activities.
    • Cooperation between nuclear and membrane T-ag mediates phenotypic transformation.
    • T-ag's interaction with p53 is critical for SV40-induced transformation.

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