Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence for trans splicing in trypanosomes.

R E Sutton, J C Boothroyd

    Cell
    |November 21, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Trypanosome messenger RNAs (mRNAs) are synthesized using trans splicing. A small RNA molecule (medRNA) provides the 5' mini-exon, which is spliced to protein-coding exons, producing mature mRNA and a free minRNA product.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Identification and characterization of HIV positive Ethiopian elite controllers in both Africa and Israel.

    HIV medicine·2018
    Same author

    Assessing the function of homologous recombination DNA repair in malignant pleural effusion (MPE) samples.

    British journal of cancer·2014
    Same author

    Transduction of CD34(+) and CD34(-)/lin(-) hemopoietic progenitors by lentivirus vectors.

    Cytotherapy·2010
    Same author

    Toxoplasma co-opts host gene expression by injection of a polymorphic kinase homologue.

    Nature·2006
    Same author

    Polymorphic secreted kinases are key virulence factors in toxoplasmosis.

    Science (New York, N.Y.)·2006
    Same author

    Infection with Toxoplasma gondii bradyzoites has a diminished impact on host transcript levels relative to tachyzoite infection.

    Infection and immunity·2006

    Area of Science:

    • Molecular Biology
    • Parasitology
    • RNA Biology

    Background:

    • Trypanosome mRNAs possess a conserved 5' mini-exon sequence.
    • This mini-exon is transcribed separately from the main protein-coding exon.

    Purpose of the Study:

    • To investigate the mechanism of mRNA synthesis in trypanosomes.
    • To test the hypothesis of trans splicing for mRNA maturation.

    Main Methods:

    • Analysis of trypanosome RNA for the presence of minRNA.
    • In vitro debranching assays using HeLa cell extracts.

    Main Results:

    • Significant quantities of free minRNA were detected in trypanosome RNA preparations.
    • minRNA could be released from larger RNA structures by a debranching enzyme.

    Related Experiment Videos

    Conclusions:

    • Trans splicing is the physiological mechanism for generating mature mRNA in trypanosomes.
    • This process involves the joining of a mini-exon from medRNA to protein-coding exons.