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MiRNA-708/CUL4B axis contributes into cell proliferation and apoptosis of osteosarcoma

G Chen1, H Zhou

  • 1Department of Orthopedic Surgery, Second Affiliated Hospital of Dalian Medical University, Dalian, China. zhouhuan8103@126.com.

European Review for Medical and Pharmacological Sciences
|September 20, 2018
PubMed
Abstract

Insights

MicroRNA-708 (miRNA-708) suppresses osteosarcoma growth by inhibiting CUL4B expression. This study reveals miRNA-708 as a potential therapeutic target for osteosarcoma, impacting cell proliferation and apoptosis.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • MicroRNA-708 (miRNA-708) has established roles in various diseases.
  • Its specific functions in osteosarcoma remain largely uncharacterized.
  • Understanding miRNA-708's role is crucial for developing new therapeutic strategies.

Purpose of the Study:

  • To investigate the role and mechanism of miRNA-708 in osteosarcoma.
  • To determine the relationship between miRNA-708 and its potential target, CUL4B.
  • To assess the impact of miRNA-708 on osteosarcoma cell behavior.

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) for miRNA-708 and CUL4B expression.
  • Cell transfection with miRNA-708 mimics and negative control.
  • Cell Counting Kit-8 (CCK-8) assay for cell proliferation.
  • Flow cytometry for apoptosis analysis.
  • Luciferase reporter assay for regulatory function confirmation.
  • Western blot for CUL4B protein expression.

Main Results:

  • MiRNA-708 expression was significantly reduced in osteosarcoma cell lines and tissues.
  • Overexpression of miRNA-708 via mimics decreased cell proliferation and increased apoptosis.
  • MiRNA-708 directly targets and downregulates CUL4B expression by binding to its 3' untranslated region (3'UTR).
  • Tumor tissues exhibited lower miRNA-708 and higher CUL4B expression, correlating with advanced stages (IIB-III).

Conclusions:

  • The miRNA-708/CUL4B axis plays a critical role in regulating osteosarcoma cell proliferation and apoptosis.
  • MiRNA-708 acts as a tumor suppressor in osteosarcoma by targeting CUL4B.
  • This axis represents a potential therapeutic target for osteosarcoma treatment.

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