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MicroRNA-337-5p participates in the development and progression of osteosarcoma via ERBB, MAPK and VEGF pathways

Z-G Tian1, Y Zhuang, Z Jin

  • 1Department of Orthopedics, The First People's Hospital of Wujiang District, Suzhou, China. zhuangyan_bmu@126.com.

European Review for Medical and Pharmacological Sciences
|September 20, 2018
PubMed
Abstract

Insights

MicroRNA-337-5p is overexpressed in osteosarcoma (OS) and promotes cancer cell growth and invasion. This microRNA is a potential diagnostic marker and therapeutic target for OS patients.

Area of Science:

  • Molecular Oncology
  • Biomarkers in Cancer

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with significant mortality.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • The specific role of microRNA-337-5p in OS remains to be fully elucidated.

Purpose of the Study:

  • To investigate the expression and function of microRNA-337-5p in osteosarcoma.
  • To explore the underlying molecular mechanisms by which microRNA-337-5p influences OS progression.
  • To evaluate microRNA-337-5p as a potential diagnostic and prognostic biomarker for OS.

Main Methods:

  • Screening of potential OS-related miRNAs using the GEO database.
  • Survival analysis, ROC curves, and correlation analysis with clinical parameters.
  • Gene Enrichment Sets Analysis (GESA) to predict pathways.
  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) for miRNA expression analysis.
  • In vitro cell proliferation (CCK-8) and invasion (Transwell) assays.
  • Western blot to investigate protein expression changes.

Main Results:

  • MicroRNA-337-5p was significantly overexpressed in OS tissues and serum samples.
  • Higher microRNA-337-5p expression correlated with poorer overall survival and larger necrosis range in OS patients.
  • GESA revealed enrichment in ERBB, MAPK, and VEGF signaling pathways.
  • Overexpression of microRNA-337-5p in OS cells increased proliferation and invasion.
  • Increased expression of ERBB2, Erk1/2, and VEGF121 was observed following microRNA-337-5p upregulation.

Conclusions:

  • MicroRNA-337-5p is upregulated in osteosarcoma and serves as an independent prognostic factor.
  • Overexpressed microRNA-337-5p promotes OS cell proliferation and invasion.
  • The oncogenic effects are mediated through the activation of ERBB, MAPK, and VEGF pathways.

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