Related Experiment Video

Updated: Feb 5, 2026

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

8.3K

EYA4 Promotes Cell Proliferation Through Downregulation of p27Kip1 in Glioma

Zhaoming Li1, Ran Qiu2, Xia Qiu3

  • 1Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|September 20, 2018
PubMed
Abstract

Insights

Eyes Absent Homologue 4 (EYA4) promotes glioma cell proliferation by suppressing p27Kip1 expression. Upregulated EYA4 correlates with advanced glioma stages and predicts poorer patient survival, highlighting its oncogenic role.

Area of Science:

  • Neuro-oncology
  • Molecular oncology
  • Cancer biology

Background:

  • Eyes Absent Homologue 4 (EYA4) is implicated in various cancers.
  • The specific role of EYA4 in glioma pathogenesis remains largely undefined.

Purpose of the Study:

  • To investigate the expression and function of EYA4 in glioma.
  • To elucidate the molecular mechanisms underlying EYA4's role in glioma development.

Main Methods:

  • Immunohistochemistry to assess EYA4 expression in glioma tissues.
  • Cell viability assays (CCK-8, BrdU) and flow cytometry to analyze cell proliferation and apoptosis.
  • Western blotting to examine protein expression levels, including p27Kip1 and Six1.

Main Results:

  • EYA4 expression is upregulated in glioma tissues and positively correlates with advanced tumor stage.
  • Higher EYA4 expression is associated with worse overall survival in glioma patients.
  • Overexpression of EYA4 enhances glioma cell proliferation by directly suppressing p27Kip1 expression, a process dependent on Six1.

Conclusions:

  • EYA4 acts as an oncoprotein in glioma, promoting cell proliferation.
  • The mechanism involves the direct suppression of p27Kip1 expression, mediated by Six1.
  • EYA4 represents a potential therapeutic target for glioma.
Keywords:
Cell proliferationEya4GliomaP27Kip1Prognosis

More Related Videos

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function06:14

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Published on: June 11, 2017

8.8K
A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG08:46

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma DIPG

Published on: March 7, 2017

17.5K

Related Concept Videos

Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.8K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.1K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

4.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.2K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.5K