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Related Experiment Videos

Expression of phosphorylcholine-specific B cells during murine development.

N H Sigal, A R Pickard, E S Metcalf

    The Journal of Experimental Medicine
    |October 1, 1977
    PubMed
    Summary

    The TEPC 15 (T15) clonotype, crucial for antibody specificity, emerges late in B-cell development, around one week after birth. Its appearance is independent of maternal factors or genetic background, suggesting a predetermined developmental pathway.

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    Area of Science:

    • Immunology
    • Developmental Biology
    • Genetics

    Background:

    • The TEPC 15 (T15) clonotype is a germline antibody specificity.
    • Its appearance in the neonatal B-cell repertoire is delayed until approximately one week of age.

    Purpose of the Study:

    • To investigate the kinetics of T15 clonotype appearance in mice.
    • To determine the influence of maternal factors and genetic background on T15 clonotype development.
    • To examine the ontogeny of phosphorylcholine (PC)-specific B cells.

    Main Methods:

    • Comparison of T15 clonotype appearance in germfree and conventionally-reared mice.
    • Analysis of T15 clonotype acquisition in neonates from different genetic backgrounds (CBF1, C57BL/6, BALB/c).
    • Assessment of PC-specific B cells in fetal liver, bone marrow, and spleen at various developmental stages.

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    Main Results:

    • The T15 clonotype appears at approximately one week of age in both germfree and conventionally-reared mice.
    • Maternal influences and genetic background have a minor role in T15 clonotype development.
    • A scarcity of PC-specific B cells and PC-binding cells is observed shortly after birth, with their emergence coinciding with spleen appearance at seven days of life.

    Conclusions:

    • The late and invariant occurrence of the T15 clonotype supports a predetermined mechanism for B-cell repertoire acquisition.
    • Findings suggest a highly ordered developmental process for B-cell specificities.
    • The study has implications for understanding antibody diversity generation.