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LncRNA-135528 inhibits tumor progression by up-regulating CXCL10 through the JAK/STAT pathway

Peng Wang1, Xiaobin Peng1, Jingjing Zhang1

  • 1The Fifth Affiliated Hospital of Southern Medical University, Guangzhou, 510900, China.

Apoptosis : an International Journal on Programmed Cell Death
|September 21, 2018
PubMed

Insights

Long non-coding RNA (lncRNA)-135528 promotes glioma cell apoptosis and inhibits proliferation. Upregulating lncRNA-135528 enhances CXCL10 levels via the JAK/STAT pathway, inhibiting tumor growth.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Spontaneous tumor regression occurs in various cancers, but the role of long non-coding RNAs (lncRNAs) in spontaneous glioma regression is understudied.
  • The specific functions and mechanisms of lncRNAs in glioma regression remain largely unknown.

Purpose of the Study:

  • To investigate the biological roles of lncRNA-135528 in spontaneous glioma regression.
  • To elucidate the potential involvement of lncRNA-135528 in regulating glioma cell behavior and associated signaling pathways.

Main Methods:

  • Obtained lncRNA-135528 cDNA using rapid-amplification of cDNA ends (RACE) technology.
  • Generated G422 glioma cells with stable lncRNA-135528 overexpression or silencing using lentiviral vectors and siRNA.
  • Assessed the effects of lncRNA-135528 manipulation on cell apoptosis, proliferation, cell cycle, CXCL10 expression, and JAK/STAT pathway components.

Main Results:

  • lncRNA-135528 overexpression promoted glioma cell apoptosis, inhibited proliferation, and arrested cell cycle progression.
  • Upregulation of lncRNA-135528 significantly increased CXCL10 levels in glioma cells.
  • lncRNA-135528 altered the expression of messenger RNAs (mRNAs) associated with the JAK/STAT signaling pathway.

Conclusions:

  • lncRNA-135528 plays a crucial role in inhibiting glioma progression.
  • The tumor-inhibitory effect of lncRNA-135528 is mediated by upregulating CXCL10 through the JAK/STAT pathway.
Keywords:
CXCL10GliomaJAK/STAT pathwaysLncRNA-135528Spontaneous tumor regression

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