Validation of optical properties quantification with a dual-step technique for biological tissue analysis

Area of Science:

• Biomedical Optics
• Optical Imaging
• Spectroscopy

Background:

• Accurate quantification of biological tissue optical properties is crucial for various imaging and diagnostic applications.
• Existing methods often struggle with wide-field assessments and heterogeneous tissue complexities.

Purpose of the Study:

• To develop and validate a novel Dual-Step setup for wide-field optical properties quantification.
• To couple diffuse reflectance spectroscopy (DRS) and multispectral imaging (MSI) for enhanced accuracy.

Main Methods:

• The Dual-Step setup integrates DRS for scattering estimation and MSI for absorption estimation.
• The ACA-Pro algorithm was adapted for both DRS and MSI for absolute quantification.
• Validation was performed using homogeneous Intralipid and heterogeneous gelatin phantoms.

Main Results:

• The Dual-Step system successfully quantified optical properties in both homogeneous and heterogeneous phantoms.
• The integration of DRS and MSI provided accurate scattering and absorption measurements.
• The adapted ACA-Pro algorithm demonstrated robust performance.

Conclusions:

• The Dual-Step setup offers a promising approach for wide-field optical property assessment in biological tissues.
• This method has potential applications in non-invasive diagnostics and imaging.
• The system's ability to handle heterogeneity is a significant advancement.