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Updated: Feb 5, 2026
A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms
Published on: January 11, 2015
Fast and environmental-friendly methods for the determination of polybrominated diphenyl ethers and their metabolites
Rebeca Cruz1, António Marques2, Susana Casal3
1LAQV/REQUIMTE, Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal.
Abstract:
Environmental-friendly, cost-effective and fast methods were developed and validated for the analysis of seven PolyBrominated Diphenyl Ethers (PBDEs) and eight methoxylated PBDEs (MeO-PBDEs) in three distinct seafood matrices (muscle, liver and plasma) and feed using a Quick, Easy, Cheap, Efficient, Rugged and Safe (QuEChERS) extraction approach for solid samples and a Dispersive Liquid-Liquid Microextraction method (DLLME) for plasma. Instrumental analyses were performed with gas chromatography coupled to triple quadrupole mass spectrometry using electron impact source (GC-EI-MS/MS) and negative ion chemical ionization (GC-NICI-MS) to assess BDE-209. Statistical validation showed recoveries for all target substances near 100% with average Relative Standard Deviation (RSD) lower than 9% and recovery standards higher than 65% (average RSD below 20%). Average calculated Method Detection Limits (MDLs) were lower than 65 pg·g-1 wet weight (WW) for muscle, 5.35 ng·g-1 WW for liver, 4.50 ng·g-1 WW for feed, and 0.60 ng·mL-1 for plasma samples. Quality assurance and quality control practices were comprehensively described. Methods scored high in an analytical Eco-scale, thus being classified as "an excellent green analysis". Finally, real seafood samples collected in local markets and local fishermen were analyzed. Positive samples presented both PBDEs and MeO-PBDEs in safe amounts (0.28-125.80 ng·g-1 WW) for human consumption.
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