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Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

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DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
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Computed Tomography01:10

Computed Tomography

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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Design Example: Traverse Angle Computations01:25

Design Example: Traverse Angle Computations

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Traverse angle computations are a critical component of surveying, used to compute the internal angles within a closed traverse. A traverse consists of a series of connected lines forming a closed loop, often used for land boundary delineation or mapping. Calculating the internal angles ensures accuracy in the traverse geometry and is essential for checking survey data integrity.The process begins with known azimuths and bearings of the traverse sides. Internal angles at each vertex are...
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Area Computation by the Alternative Coordinate Method01:24

Area Computation by the Alternative Coordinate Method

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The alternative coordinate method, also known as the Shoelace Formula, is a technique for determining the area of a traverse using Cartesian coordinates. This method relies on the sequential arrangement of x and y coordinates for each point of the shape, ensuring accuracy and ease of application.In this approach, each corner's x and y coordinates are listed as fractions, with the x-coordinate as the numerator and the y-coordinate as the denominator. These coordinates are arranged sequentially...
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The Role of Ion Channels in Neuronal Computation01:19

The Role of Ion Channels in Neuronal Computation

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A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
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Related Experiment Video

Updated: Feb 5, 2026

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
08:01

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

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Computational studies on Begomoviral AC2/C2 proteins.

Kanthalu Shagadevan Dinesh Babu1, Prabu Manoharan2,3, Gopal Pandi1

  • 1Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.

Bioinformation
|September 22, 2018
PubMed
Summary

Begomoviruses, a type of Geminiviridae, use the AC2/C2 protein to infect crops. Sequence analysis reveals differences in AC2/C2 structure, suggesting a mechanism for host gene activation crucial for viral infection.

Keywords:
AC2 proteinCLE elementsGemini virusHost- gene transactivationTranscriptional activator proteinacidic activation domainprotein interaction

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Area of Science:

  • Plant virology
  • Molecular biology
  • Genomics

Background:

  • Geminiviridae are significant plant pathogens causing crop diseases.
  • Begomoviruses within this family possess a pathogenic AC2/C2 protein essential for infection.

Purpose of the Study:

  • To analyze the C-terminal region of the begomoviral AC2/C2 protein.
  • To understand the structural and functional implications of AC2/C2 in host gene transactivation.

Main Methods:

  • Sequence analysis of AC2/C2 proteins from bipartite and monopartite begomoviruses.
  • Secondary structure prediction of the AC2/C2 C-terminal domain.
  • Genome-wide mapping of the conserved late element (CLE) in Arabidopsis thaliana.
  • Protein interaction network analysis.

Main Results:

  • Significant variations in protein length, composition, and residue positions were observed in AC2/C2.
  • The C-terminal region of AC2/C2 may form an alpha-helix, similar to known transcriptional activators.
  • 122 Arabidopsis thaliana promoters contained the exact CLE consensus sequence.
  • Protein interaction analysis indicated potential regulation of hundreds of host proteins by AC2.

Conclusions:

  • The C-terminal region of AC2/C2 plays a critical role in begomovirus pathogenesis.
  • Begomoviruses likely manipulate host cellular machinery through AC2/C2 to facilitate infection.
  • Understanding AC2/C2 function offers targets for developing crop protection strategies.