Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple C4/Slp genes distinguished by expression after transfection.

D M Robins, M Malissen, L Hood

    Molecular and Cellular Biology
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Automated extraction of temporalized tumor evolution from oncology EMRs using natural language processing.

    ESMO real world data and digital oncology·2026
    Same author

    Laminaria tent use for dilation, extraction, and curettage leading to septic shock: a case report.

    Journal of medical case reports·2025
    Same author

    Biometric and biochemical characterization for potential uses of Euterpe edulis (Martius) seeds.

    Brazilian journal of biology = Revista brasleira de biologia·2025
    Same author

    Variation in the diet composition and weight-length relationship of small characids in urbanized and forested streams.

    Journal of fish biology·2025
    Same author

    Physiological quality of seeds of arabic coffee cultivars stored for a period of two years.

    Brazilian journal of biology = Revista brasleira de biologia·2024
    Same author

    Potential of coffee straw biochal as a substrate conditioner in seed lettuce and sorghum germination and vigority.

    Brazilian journal of biology = Revista brasleira de biologia·2024

    The murine S region contains complement component 4 (C4) and sex-limited protein (Slp) genes. Researchers identified one C4 and at least three Slp-like genes in the B10.W7R strain, with functional expression observed after gene transfection.

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Complement System

    Background:

    • The murine S region of the major histocompatibility complex harbors C4 and Slp genes.
    • The B10.W7R strain exhibits unique characteristics regarding Slp protein androgen regulation.

    Purpose of the Study:

    • To clone and characterize C4 and Slp genes from the B10.W7R murine strain.
    • To investigate the gene copy number and evolutionary amplification within the Sw7 locus.
    • To assess the functional expression of cloned genes after transfection into L cells.

    Main Methods:

    • Cosmid library cloning of S region genes.
    • Restriction site polymorphism analysis to determine gene copy number.
    • Gene transfection into L cells for expression analysis.

    Related Experiment Videos

    Main Results:

    • Identified at least four C4-like genes within the Sw7 locus, suggesting gene duplication.
    • Transfected C4 and Slp genes were expressed, processed, and secreted correctly in L cells.
    • Demonstrated functional expression of at least one C4 gene and two Slp genes, producing proteins similar to macrophage-derived ones.

    Conclusions:

    • The B10.W7R S region comprises one C4 gene and a minimum of three Slp-like genes.
    • Gene amplification has occurred within this region.
    • Transfection studies confirm the functional capacity of identified genes to produce immunologically active C4 and Slp proteins.