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Identification of the anabolic steroid 6β-chlorotestosterone in a dietary supplement
Travis M Falconer1, Rick A Flurer1, Mary B Jones1
1US Food and Drug Administration, Forensic Chemistry Center, Cincinnati, OH, 45237, USA.
Abstract:
Capsules that were labeled to be performance-enhancing dietary supplements obtained during an investigation were found to contain an unrecognized steroid-like substance. This compound was isolated by liquid chromatography (LC) fraction collection and characterized using several qualitative analytical techniques, including ultraviolet (UV) spectroscopy, gas chromatography-mass spectrometry (GC-MS), liquid chromatography-high resolution accurate mass-mass spectrometry (LC-HRAM-MS), as well as 1 H, 13 C, and two-dimensional nuclear magnetic resonance (NMR) spectrometry. This multi-technique analytical approach was used to identify the designer steroid as 6β-chloro-4-androsten-17β-ol-3-one (6β-chlorotestosterone), an analog of testosterone about which little has been published.
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