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Holding immature equine oocytes for up to 3 nights prior to ICSI under practical shipping conditions (10-22 °C) is compatible with blastocyst development.

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Reproductive Techniques for Ovarian Monitoring and Control in Amphibians
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Assisted reproductive techniques in mares.

Katrin Hinrichs1

  • 1Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas.

Reproduction in Domestic Animals = Zuchthygiene
|September 22, 2018
PubMed
Summary

Assisted reproductive techniques (ARTs) like oocyte recovery and intracytoplasmic sperm injection (ICSI) offer efficient solutions for equine breeding. These methods aid in managing subfertility, preserving genetics, and increasing foal production in horses.

Keywords:
cloningcryopreservationembryoshorsesintracytoplasmicorganismpreimplantation diagnosissperm injections

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Area of Science:

  • Equine reproduction
  • Assisted reproductive technologies

Background:

  • A variety of assisted reproductive techniques (ARTs) are available for equine reproduction management.
  • These techniques assist in obtaining multiple foals per mare annually and from mares unable to carry a pregnancy to term.

Purpose of the Study:

  • To outline the applications and advancements in assisted reproductive techniques for horses.
  • To highlight the efficiency and accessibility of oocyte recovery and intracytoplasmic sperm injection (ICSI) in equine reproduction.

Main Methods:

  • Oocyte recovery from live or deceased mares for subsequent in vitro fertilization (IVF) or ICSI.
  • Embryo culture, biopsy for genetic analysis, and cryopreservation (vitrification) of embryos.
  • Nuclear transfer (cloning) for genetic preservation.

Main Results:

  • Intracytoplasmic sperm injection (ICSI) is effective for subfertile mares and cases with low sperm quality.
  • Oocyte recovery and ICSI can be performed efficiently, even with overnight shipping of oocytes.
  • Successful vitrification of equine embryos is achievable, while cryopreservation of oocytes remains challenging.
  • Nuclear transfer is a clinically available service for preserving equine genetics.

Conclusions:

  • Oocyte recovery and ICSI are increasingly used for managing both subfertile and normally fertile horses due to efficiency.
  • Advancements in embryo cryopreservation and nuclear transfer offer significant tools for equine genetic preservation and breeding programs.