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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

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Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
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Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

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Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
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Quality Control01:05

Quality Control

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Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
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Quality Assurance01:19

Quality Assurance

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Quality assurance is the overarching term used to describe the activities employed to ensure the proper performance of a system. These activities can be classified into three categories: quality control, quality assessment, and internal corrective measures. Typically, these activities work cyclically: quality control is performed before and during the analysis, while quality assessment occurs during and after the investigation. Internal corrective measures are implemented based on the findings...
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Related Experiment Video

Updated: Feb 5, 2026

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
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Parkour LIMS: high-quality sample preparation in next generation sequencing.

E Anatskiy1, D P Ryan2, B A Grüning1,3

  • 1Bioinformatics Group, Department of Computer Science, University of Freiburg, Freiburg, Germany.

Bioinformatics (Oxford, England)
|September 22, 2018
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Summary

Parkour is a new software package designed to streamline next-generation sequencing sample processing and quality management. It enhances laboratory efficiency and reduces turnaround times for sequencing services.

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Area of Science:

  • Bioinformatics
  • Genomics
  • Laboratory Management

Background:

  • Next-generation sequencing (NGS) generates large volumes of data requiring robust sample processing and quality control.
  • Academic core laboratories face challenges in managing sample workflows, tracking quality, and optimizing efficiency.

Purpose of the Study:

  • To introduce Parkour, a software package for comprehensive sample processing and quality management in NGS workflows.
  • To enhance efficiency, reduce turnaround times, and minimize administrative tasks in core laboratories.

Main Methods:

  • Parkour is a web-based application that tracks samples through preparation stages.
  • It assesses samples against predefined quality criteria.
  • Features include intelligent sample grouping, staff assignment, automated invoicing, usage statistics, and report generation.

Main Results:

  • Parkour enables efficient tracking and quality assessment of NGS samples.
  • It optimizes laboratory operations through intelligent sample grouping and work unit assignment.
  • Automated administrative tools reduce workload and improve service delivery.

Conclusions:

  • Parkour offers a flexible and efficient solution for managing NGS sample processing and quality control.
  • Its web-based nature and APIs facilitate integration and data sharing.
  • The software is suitable for academic core laboratories and NGS service providers.