Jove
Visualize
Contact Us

Related Concept Videos

Meristems and Plant Growth02:36

Meristems and Plant Growth

49.5K
Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
49.5K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

15.1K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
15.1K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

4.5K
4.5K
Primary and Secondary Growth in Roots and Shoots03:02

Primary and Secondary Growth in Roots and Shoots

60.5K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.5K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

8.5K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.5K
Binet's Contribution to Measures of Intelligence01:23

Binet's Contribution to Measures of Intelligence

1.7K
Alfred Binet, along with his student Théophile Simon, was tasked by the French Ministry of Education in 1904 to create a method for identifying students who struggled to learn through conventional classroom instruction. This initiative aimed to address overcrowding by placing such students in specialized schools. Binet and Simon developed an intelligence test comprising 30 tasks, ranging from simple commands, like touching one's nose or ear, to more complex tasks, such as drawing...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasmodesmata display dynamic local and systemic redox responses during plant stress.

The Plant cell·2026
Same author

Fortified farm yard manure to bio-stimulate nutrient availability and production of Indian mustard.

PloS one·2026
Same author

Multi-environment phenotyping of ricebean (<i>Vigna umbellata</i> (Thunb.) Ohwi & Ohashi) germplasm and identification of core set for accelerating the crop improvement programs.

Frontiers in plant science·2026
Same author

CBL1/9-CIPK6 complex negatively regulates Respiratory burst oxidase homolog D in Arabidopsis thaliana.

The Plant journal : for cell and molecular biology·2026
Same author

Intraluminal Vesicles as Transfection Intermediaries.

Pharmaceutics·2025
Same author

Unearthing the secrets of drought-driven root system architecture: Nutrient acquisition and rhizosphere microbe interplay.

Plant science : an international journal of experimental plant biology·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 5, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

19.3K

CBL-interacting protein kinase 25 contributes to root meristem development.

Mukesh Kumar Meena1, Niraj Kumar Vishwakarma1, Vineeta Tripathi1

  • 1National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.

Journal of Experimental Botany
|September 22, 2018
PubMed
Summary

Calcineurin B-like protein interacting protein kinase 25 (CIPK25) regulates root meristem size by coordinating auxin and cytokinin signaling. Loss of CIPK25 impairs root growth, while its restoration rescues the phenotype.

More Related Videos

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

11.8K
Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
06:46

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

Published on: March 29, 2019

13.1K

Related Experiment Videos

Last Updated: Feb 5, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

19.3K
Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

11.8K
Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species
06:46

Confocal Live Imaging of Shoot Apical Meristems from Different Plant Species

Published on: March 29, 2019

13.1K

Area of Science:

  • Plant biology
  • Molecular signaling
  • Developmental biology

Background:

  • Root meristem size is crucial for plant development and is regulated by hormonal signals.
  • Calcium signaling pathways, involving Calcineurin B-like proteins (CBLs) and their interacting protein kinases (CIPKs), play roles in relaying cellular signals.

Purpose of the Study:

  • To investigate the role of CIPK25 in regulating root meristem size and its interaction with auxin and cytokinin signaling pathways.

Main Methods:

  • Arabidopsis thaliana genetic analysis (T-DNA insertion lines, loss-of-function mutations).
  • Phenotypic analysis of root growth and meristem size.
  • Gene expression analysis (RT-qPCR, promoter activity assays).
  • Protein-protein interaction studies.

Main Results:

  • Arabidopsis plants lacking CIPK25 exhibited a short root phenotype with reduced meristem cells, which was rescued by CIPK25 expression.
  • CIPK25 interacts with CBL4 and CBL5, and its expression is regulated by auxin and cytokinin.
  • The cipk25 mutant showed defects in auxin transport and altered expression of auxin efflux carriers (PIN1, PIN2) and SHY2.
  • Restoring SHY2 function rescued the decreased PIN1 and PIN2 expression and root growth defects in the cipk25 mutant.

Conclusions:

  • CIPK25 is essential for coordinating auxin and cytokinin signaling to control root meristem size during plant development.
  • CIPK25 influences auxin transport and the expression of key genes involved in root growth regulation.
  • A CIPK25-independent cytokinin signaling pathway also contributes to the regulation of SHY2 and PIN1 expression.