Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutational alterations induced in yeast by ionizing radiation.

G Das, J W Stewart, F Sherman

    Mutation Research
    |December 1, 1986
    PubMed
    Summary

    Ionizing radiation, like X-rays, causes random DNA base-pair substitutions in yeast. While mostly single changes, some complex mutations and site-specific prohibitions were observed.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genomic and immunoinformatic characterization of canine adenovirus type 1 in parvovirus-infected dogs: A comprehensive study from northeast India.

    Polish journal of veterinary sciences·2026
    Same author

    Integrative machine learning approaches with genomic data for predicting antitubercular drug resistance: A systematic review and meta-analysis.

    Journal of global antimicrobial resistance·2025
    Same author

    Enhancement of solar cell efficiency through tailored electrodeposited seed layers and CdS: O surface texturing.

    Scientific reports·2025
    Same author

    Predicting piglet survival until weaning using birth weight and within-litter birth weight variation as easily measured proxy predictors.

    Animal : an international journal of animal bioscience·2025
    Same author

    Prevalence of Crimean-Congo Haemorrhagic Fever virus and Ganjam virus among livestock & ticks in Wayanad, Kerala.

    Veterinary research communications·2024
    Same author

    Anisotropic atomic displacements, local orthorhombicity and anomalous local magnetic moment in Ba<sub>0.6</sub>K<sub>0.4</sub>Fe<sub>2</sub>As<sub>2</sub> superconductor.

    Physical chemistry chemical physics : PCCP·2024

    Area of Science:

    • Molecular Biology
    • Radiation Biology
    • Yeast Genetics

    Background:

    • Understanding DNA mutation mechanisms is crucial for radiation biology.
    • The yeast Saccharomyces cerevisiae is a model organism for studying mutagenesis.

    Purpose of the Study:

    • To investigate the types of DNA mutations induced by ionizing radiation (X-rays and alpha-particles).
    • To characterize the base-pair substitutions and complex changes resulting from radiation exposure in yeast.

    Main Methods:

    • Analysis of revertants from yeast cycl-9 (UAA) and cycl-179 (UAG) mutants.
    • Amino acid analysis, peptide mapping, and partial amino acid sequencing of iso-1-cytochromes c.
    • Cloning and sequencing of DNA segments with complex mutations.

    Main Results:

    • Ionizing radiation primarily induces single base-pair substitutions (transitions and transversions) with no apparent preference.
    • A specific base-pair substitution (A x T -> T x A) was notably absent, suggesting site-specific mutation prohibitions.
    • Five revertants exhibited multiple changes within a small DNA region, including deletions and multiple substitutions.

    Conclusions:

    • Ionizing radiation induces a broad spectrum of base-pair substitutions, appearing more random than other mutagens.
    • Evidence suggests that certain DNA sites may have restrictions on specific base-pair substitutions.
    • Complex mutations, including deletions and multiple substitutions, can occur in localized DNA regions.

    Related Experiment Videos