RecG_wed : A probable novel regulator in the resolution of branched DNA structures in mycobacteria

Structure-specific helicases, such as RecG, play an important role in the resolution of recombination intermediates. A bioinformatic analysis of mycobacterial genomes led to the identification of a protein (RecG_wed ) with a C-terminal "edge" domain, similar to the wedge domain of RecG. RecG_wed is predominately found in the phylum Actinobacteria and in few human pathogens. Mycobacterium smegmatis RecG_wed was able to bind branched DNA structures in vitro but failed to interact with single- or double-stranded DNA. The expression of recG_wed in M. smegmatis cells was up-regulated during stationary phase/UV damage and down-regulated during MMS/H₂ O₂ treatment. These observations indicate the possible involvement of RecG_wed in transactions during recombination events, that proceed though branched DNA intermediates. © 2018 IUBMB Life, 70(8):786-794, 2018.