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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

58.9K

Circulating monocyte subsets and heart failure prognosis

Elena Elchinova1,2,3, Iris Teubel4, Santiago Roura5,6

  • 1Heart Failure Unit and Cardiology Service, Germans Trias i Pujol University Hospital, Badalona, Spain.

Plos One
|September 22, 2018
PubMed

Insights

The absolute count of intermediate monocytes, not their percentage, predicts worse outcomes in heart failure (HF) patients. Higher intermediate monocyte cell counts indicate increased risk for death and composite endpoints in ambulatory HF.

Area of Science:

  • Immunology
  • Cardiology
  • Clinical Research

Background:

  • Monocytes are crucial immune cells involved in tissue repair.
  • Heart failure (HF) prognosis can be influenced by immune cell dynamics.
  • Understanding monocyte subset roles is vital for HF patient management.

Purpose of the Study:

  • To investigate the prognostic significance of monocyte subsets in ambulatory heart failure patients.
  • To determine whether monocyte percentage or absolute count is a better predictor of adverse outcomes in HF.
  • To identify specific monocyte subsets associated with mortality and hospitalization in HF.

Main Methods:

  • Classification of monocyte subsets: classical (CD14++/CD16-), intermediate (CD14++/CD16+), and non-classical (CD14+/CD16++).
  • Assessment of percentage distribution and absolute cell counts for each subset.
  • Multivariable Cox regression analyses to evaluate associations with all-cause death, HF hospitalization, and composite endpoints in 400 HF patients.

Main Results:

  • Monocyte subsets as percentages were not independently associated with any endpoints.
  • Absolute cell count of the intermediate monocyte subset showed independent association with increased all-cause death (HR 1.25) and composite endpoints (HR 1.20).
  • Follow-up of 2.6 years revealed significant differences in outcomes based on intermediate monocyte counts.

Conclusions:

  • Absolute monocyte counts, specifically for the intermediate subset, are superior to percentages for prognostic stratification in ambulatory HF patients.
  • The intermediate monocyte subset serves as a valuable biomarker for increased risk of mortality and composite events in HF.
  • This finding aids in refining risk assessment and potentially guiding therapeutic strategies for HF management.

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