Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic01:26

Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic

5.6K
Healthcare-associated infections (HAIs) occur in a healthcare facility while a person receives care for another ailment. This category also includes work-related infections among healthcare staff.
HAIs significantly increase the cost of health care. Extended stays in healthcare institutions, increased disability, increased costs of medications, including specialized antibiotics, and prolonged recovery times add to the patient's expenses and the healthcare institution and funding bodies.
5.6K
Labeling Emotion01:20

Labeling Emotion

731
Emotional labeling is a cognitive process that involves identifying and naming one's emotions, such as anger, fear, happiness, or sadness. It allows individuals to recognize and express their internal emotional states, a critical aspect of emotional regulation and communication. Labeling emotions requires more than mere recognition; it also involves drawing upon memory and contextual cues to understand the current situation and apply a corresponding emotional label. For instance, feeling...
731
Labeling DNA Probes03:31

Labeling DNA Probes

9.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.4K
Tissues01:18

Tissues

85.4K
Cells with similar structure and function are grouped into tissues. A group of tissues with a specialized function is called an organ. There are four main types of tissue in vertebrates: epithelial, connective, muscle, and nervous.
85.4K
Tissues01:25

Tissues

68.1K
Tissues are a group of cells that share a common embryonic origin. Microscopic observation reveals that the cells in a tissue share morphological features and are arranged in an orderly pattern to perform specific functions. From an evolutionary perspective, tissues appear in more complex organisms. Although there are many types of cells in the human body, they are organized into four broad categories of tissues: epithelial, connective, muscle, and nervous. Each of these categories is...
68.1K
Plant Cells and Tissues02:01

Plant Cells and Tissues

65.7K
Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
65.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ischemic injury triggers a protective microglial phenotype in models of Aβ pathology.

Journal of neuroinflammation·2026
Same author

Soluble epoxide hydrolase drives neurovascular dysfunction in a model of amyloidosis.

Brain : a journal of neurology·2026
Same author

Alzheimer's Disease Co-Pathology and Cognitive Impairment in Amyotrophic Lateral Sclerosis.

Annals of neurology·2026
Same author

Glucose hypometabolism and hyperphosphorylated Tau synergistically drive neuronal necroptosis.

Neuron·2026
Same author

Type I interferon drives T cell responses to amyloid beta in the central nervous system.

Nature communications·2026
Same author

Microglia-glioblastoma crosstalk mediates glioblastoma invasion at the far infiltration zone.

Immunity·2026
Same journal

Topological skeleton analysis for network-based shape representation in biology and beyond.

iScience·2026
Same journal

Condition-specific neural signatures of reactivation during post-retrieval rest: An EEG study.

iScience·2026
Same journal

Multi-chaotic signal identification employing a causal cross-correlation neural network.

iScience·2026
Same journal

Repeated insertions at positions 261-280 in KPC-2 highlight a ceftazidime-avibactam resistance hotspot.

iScience·2026
Same journal

ROS inhibits microtubule dynamics and cell growth heterogeneity during Arabidopsis sepal morphogenesis.

iScience·2026
Same journal

Type 1 diabetes alters early macrophage-<i>Mycobacterium tuberculosis</i> transcriptional coordination during infection.

iScience·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

Author Spotlight: Learning Systematic Bronchoscopy in a Simulation-Base Setting
04:47

Author Spotlight: Learning Systematic Bronchoscopy in a Simulation-Base Setting

Published on: June 23, 2023

3.5K

Label-free 3D-CLEM Using Endogenous Tissue Landmarks.

Manja Luckner1, Steffen Burgold2, Severin Filser3

  • 1Department of Biology I, Biocenter Ludwig-Maximilians-University Munich, Planegg-Martinsried 82152, Germany; German Center for Neurodegenerative Diseases (DZNE), Translational Brain Research, Munich 81377, Germany.

Iscience
|September 22, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a new brain tissue preparation method for high-precision microscopy. It uses natural landmarks, avoiding artificial markers to better study neuronal structure and function.

Keywords:
Biological Sciences Research MethodologiesBiological Sciences ToolsNeuroscienceTechniques in Neuroscience

More Related Videos

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
09:51

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Published on: December 13, 2017

16.2K
Tissue Engineering of a Human 3D in vitro Tumor Test System
11:12

Tissue Engineering of a Human 3D in vitro Tumor Test System

Published on: August 6, 2013

21.8K

Related Experiment Videos

Last Updated: Feb 5, 2026

Author Spotlight: Learning Systematic Bronchoscopy in a Simulation-Base Setting
04:47

Author Spotlight: Learning Systematic Bronchoscopy in a Simulation-Base Setting

Published on: June 23, 2023

3.5K
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
09:51

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Published on: December 13, 2017

16.2K
Tissue Engineering of a Human 3D in vitro Tumor Test System
11:12

Tissue Engineering of a Human 3D in vitro Tumor Test System

Published on: August 6, 2013

21.8K

Area of Science:

  • Neuroscience
  • Microscopy
  • Cell Biology

Background:

  • 3D correlative light and electron microscopy (CLEM) is crucial for understanding neuronal structure-function relationships.
  • Current CLEM protocols often use artificial fiducial markers, risking obscuring or damaging the biological structures of interest.

Purpose of the Study:

  • To develop a universally applicable sample preparation method for high-precision overlay of light and scanning electron microscopy images.
  • To enable CLEM without artificial fiducial markers by utilizing endogenous brain landmarks.

Main Methods:

  • A novel "flat embedding" preparation technique was developed for brain tissue.
  • High-precision overlay was achieved using endogenous landmarks: blood vessels, nuclei, and myelinated axons.
  • The workflow was validated by combining in vivo 2-photon microscopy and focused ion beam scanning electron microscopy (FIB-SEM).

Main Results:

  • The flat embedding method allows precise correlation of light and scanning electron micrographs.
  • Endogenous landmarks effectively replace artificial fiducial markers, preserving structural integrity.
  • The technique was successfully applied to investigate the role of astrocytic coverage in dendritic spine persistence.

Conclusions:

  • The reported flat embedding preparation offers a generalizable and non-invasive approach for advanced CLEM in neuroscience.
  • This method enhances the study of neuronal structure-function dynamics by eliminating the need for artificial markers.
  • The findings provide new insights into the role of astrocytes in synaptic plasticity and stability.