Related Experiment Video
Updated: Feb 5, 2026

Reinstatement of Drug-seeking in Mice Using the Conditioned Place Preference Paradigm
Published on: June 7, 2018
Geranylgeranylacetone blocks the reinstatement of morphine-conditioned place preference
Ningning Guo1, Xianwen Zhang1, Mengbing Huang1
1Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China; Medical Faculty, Kunming University of Science and Technology, Kunming, 650500, China.
Abstract:
Morphine is widely used for clinical pain management and induces the dependence. Addiction to morphine is a major public health issue. Geranylgeranylacetone (GGA) is widely used in clinic for treating ulcer. GGA induces expression of thioredoxin-1 (Trx-1) extensively. Trx-1 is a redox regulating protein and plays protecting roles in nervous system. GGA prevents mice against morphine-induced hyperlocomotion, rewarding effect, and withdrawal syndrome. However, whether GGA blocks morphine-conditioned place preference (CPP) reinstatement is still unknown. In the present study, we found that GGA administration blocked the reinstatement of morphine-CPP. The expressions of Trx-1, N-methyl d-aspartate receptor 2B subunit (NR2B), phosphorylated Ca2+/calmodulin-dependent protein kinase II (p-CaMKII), phosphorylated extracellular signaling regulated kinases (p-ERK), and phosphorylated cAMP-response element binding protein (p-CREB) were induced in nucleus accumbens (NAc) and hippocampus by morphine or GGA, whereas these proteins were not changed by morphine in GGA-treated mice. Our results indicate that GGA may prevent the reinstatement of morphine-CPP through strengthening the expression of Trx-1 and regulating NR2B/ERK pathway. Thus, we suggest that GGA may be a promising therapeutic candidate for morphine-induced relapse.
More Related Videos
04:11A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats
Published on: November 6, 2018
08:22Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
Published on: July 30, 2013
Related Concept Videos
Opioid Analgesics: Morphine and Other Natural Cogeners
Block Diagram Reduction
The first step in this process is the identification and relocation of a branch point. A branch point, where a...
Elements of Block Diagrams
A block diagram typically includes essential elements such as comparators, blocks, and feedback loops. Each of these elements...
Natural Selection and Mating Preferences
Females, due to their biological roles in conception, pregnancy, and nursing,...
Conditions on Early Earth
Classical Conditioning
Ivan Pavlov observed that dogs...