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Synthesis of Bimetallic Pt/Sn-based Nanoparticles in Ionic Liquids
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Isolation of C-phycocyanin from Spirulina platensis microalga using Ionic liquid based aqueous two-phase system
Yu-Kaung Chang1, Pau-Loke Show2, John Chi-Wei Lan3
1Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan.
Bioresource Technology
|September 22, 2018
Summary
Ionic liquids in aqueous two-phase systems efficiently isolate C-phycocyanin from microalgae. This study optimizes parameters for high extraction efficiency, demonstrating a spontaneous and effective bioseparation process.
Area of Science:
- Biotechnology
- Biochemistry
- Chemical Engineering
Background:
- C-phycocyanin (CPC) is a valuable phycobiliprotein with diverse applications.
- Efficient and scalable isolation methods for CPC from microalgae like Spirulina platensis are crucial.
- Aqueous two-phase systems (ATPS) offer a promising platform for bioseparation.
Purpose of the Study:
- To investigate the use of ionic liquids (ILs) within ATPS for isolating C-phycocyanin from Spirulina platensis.
- To evaluate the impact of various ILs and experimental parameters on CPC extraction efficiency.
- To determine the optimal conditions for maximizing CPC recovery and purity.
Main Methods:
- Utilized an aqueous two-phase system (ATPS) incorporating various imidazolium-based ionic liquids and potassium salts.
- Systematically varied experimental parameters including pH, temperature, loading volume, algae concentration, and IL alkyl chain length.
- Analyzed CPC extraction efficiency, partition coefficient, and separation factor under different conditions.
Main Results:
- Achieved a maximum extraction efficiency of 99% for CPC.
- Identified optimal conditions at pH 7 and 308 K, yielding a partition coefficient of 36.6 and a separation factor of 5.8.
- Demonstrated that 1-octyl-3-methylimidazolium bromide (C8MIM-Br) provided superior extraction efficiency compared to shorter-chain analogues (C6MIM-Br, C4MIM-Br).
Conclusions:
- Ionic liquid-based ATPS is a highly effective method for C-phycocyanin isolation from Spirulina platensis.
- The isolation process adheres to pseudo second-order kinetic models, indicating a spontaneous thermodynamic pathway.
- Optimized IL-ATPS offers a scalable and efficient approach for recovering valuable CPC from microalgal biomass.
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