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Nanosilicate embedded agarose hydrogels with improved bioactivity.

Fuat Topuz1, Ali Nadernezhad2, Ozum S Caliskan2

  • 1Faculty of Engineering and Natural Sciences, Sabanci University, 34956 Tuzla, Istanbul, Turkey.

Carbohydrate Polymers
|September 23, 2018
PubMed
Summary

This study synthesized nanocomposite agarose hydrogels using nanosilicates (Laponite) to enhance cell activity. The resulting materials show improved mechanical properties and promote fibroblast cell proliferation and spreading.

Keywords:
AgaroseBioactive hydrogelsLaponiteNanocompositeNanosilicates

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Area of Science:

  • Biomaterials Science
  • Materials Chemistry
  • Nanotechnology

Background:

  • Agarose hydrogels are widely used in biomedical applications.
  • Enhancing the bioactivity and mechanical properties of agarose hydrogels is crucial for tissue engineering.
  • Anisotropic 2D nanosilicates offer potential for material property modification.

Purpose of the Study:

  • To synthesize nanocomposite agarose hydrogels incorporating anisotropic 2D nanosilicates (Laponite).
  • To investigate the effect of nanosilicate incorporation on the physicochemical and mechanical properties of agarose hydrogels.
  • To evaluate the impact of these nanocomposite hydrogels on NIH/3T3 fibroblast cell behavior.

Main Methods:

  • Synthesis of nanocomposite agarose hydrogels with varying nanosilicate content.
  • Rheological measurements to determine gelation temperature and mechanical properties.
  • EDX and FTIR analysis to confirm nanosilicate presence and interactions.
  • Cell culture studies to assess fibroblast proliferation and spreading.

Main Results:

  • Nanosilicates slightly increased the gelation temperature of agarose hydrogels.
  • Higher nanosilicate content improved the mechanical properties of the gels.
  • Nanosilicates did not significantly alter swelling properties but caused agarose nanofiber collapse.
  • EDX and FTIR confirmed nanosilicate integration and physical interactions with agarose.
  • Encapsulated NIH/3T3 fibroblast cells exhibited enhanced proliferation and spreading.

Conclusions:

  • Nanocomposite agarose hydrogels with incorporated nanosilicates demonstrate enhanced bioactivity.
  • These materials show improved mechanical strength and promote cell growth.
  • The findings suggest potential applications in regenerative medicine and tissue engineering.