Related Experiment Video
Updated: Feb 5, 2026

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
Published on: September 15, 2016
Chitosan and carboxymethyl cellulose-multilayered magnetic fluorescent systems for reversible protein immobilization
Lei Li1, Feijun Wang1, Ziqiang Shao1
1Beijing Engineering Research Centre of Cellulose and Its Derivatives, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing, 100081, China.
Abstract:
Magnetic fluorescent nanoparticles (MFNPs)-based protein immobilization systems were successfully synthesized via a layer-by-layer (LbL) assembly approach in an impinging stream-rotating packed bed, which adopted chitosan containing carbon dots (CDs)@Fe3O4 nanoparticles (NPs) as a precursor, chitosan (CS) or carboxymethylcellulose (CMC) involving CDs as shells. To reveal the relationship between structure and efficiency of systems, the effect of self-assembly mode and layer number\was investigated to provide us insight into how to improve the design of MFNPs-based supports. Finally, an MFNPs-based protein immobilization system with excellent fluorescence and magnetic response, expanded specific surface area, and enhanced immobilization and release performance were obtained. Immobilization mechanism and kinetic studies were also conducted to reveal the rate-controlling step and the template affinity. Taken together, this study provides an effective strategy to synthesize bifunctional MFNPs and to adjust the performance, which aims for facilitating new possibilities for biomass-based nanomaterials used for protein immobilization.
More Related Videos
11:13Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Published on: August 20, 2018
14:43Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
Published on: September 23, 2013
Related Concept Videos
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the...
Gram-negative Bacterial Protein Secretion Systems
Second Order systems II
Reversible and Irreversible Processes
Diode: Reverse bias
Colors and Magnetism
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...