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Circ-SATB2 upregulates STIM1 expression and regulates vascular smooth muscle cell proliferation and differentiation

Yan-Yang Mao1, Jun-Qiang Wang2, Xiao-Xian Guo3

  • 1Department of Cardiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China; Department of Cardiology, The Affiliated Hospital of Yan'an University, Yan'an 716000, China.

Biochemical and Biophysical Research Communications
|September 23, 2018
PubMed

Insights

Circular RNAs (circRNAs) like circ-SATB2 are crucial in coronary heart disease (CHD). Circ-SATB2 promotes STIM1 expression, impacting vascular smooth muscle cell (VSMC) function and potentially contributing to CHD development.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Epigenetics

Background:

  • Coronary heart disease (CHD) remains a significant clinical challenge.
  • Circular RNAs (circRNAs) are increasingly recognized for their roles in disease pathogenesis.
  • Vascular smooth muscle cells (VSMCs) are key players in the development of CHD.

Purpose of the Study:

  • To investigate the role of circ-SATB2 in regulating VSMC function.
  • To elucidate the molecular mechanism by which circ-SATB2 influences VSMC behavior.
  • To explore the potential of circ-SATB2 as a therapeutic target for CHD.

Main Methods:

  • Detection of circRNA, miRNA, and mRNA expression in VSMCs.
  • Overexpression and knockdown experiments for circ-SATB2 and miR-939.
  • Analysis of VSMC phenotypic differentiation, proliferation, migration, and apoptosis.
  • Co-transfection studies to assess molecular interactions.

Main Results:

  • Circ-SATB2 and STIM1 were upregulated, while miR-939 was downregulated in proliferative VSMCs.
  • Circ-SATB2 promotes STIM1 expression and enhances VSMC proliferation and migration.
  • Circ-SATB2 inhibits SM22α expression, a marker of contractile VSMCs.
  • MiR-939 inhibits STIM1 expression and promotes VSMC apoptosis.
  • Circ-SATB2 appears to regulate VSMC function primarily through STIM1.

Conclusions:

  • Circ-SATB2 plays a critical role in regulating VSMC phenotypic differentiation, proliferation, apoptosis, and migration.
  • The mechanism involves circ-SATB2 promoting STIM1 expression.
  • These findings offer a theoretical basis for understanding circRNA's role in VSMCs and CHD pathogenesis.
Keywords:
AtherosclerosisCoronary artery diseaseVascular smooth muscle cellcircRNAmiR-939

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