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Updated: Feb 5, 2026

Characterizing Extracellular Vesicles from Biological Fluids
Published on: February 28, 2025
Extracellular vesicles: translational challenges and opportunities
Hannah Clemmens1, Daniel W Lambert2,3
1Academic Unit of Oral and Maxillofacial Pathology, School of Clinical Dentistry, University of Sheffield, S10 2TA Sheffield, U.K. hsclemmens1@sheffield.ac.uk.
Abstract:
Extracellular vesicles (EVs) are a heterogeneous group of small lipid-enclosed structures with myriad roles in physiology and disease. The recent surge of interest in EVs has led to greater understanding of their biology and appreciation of how they might be utilised as diagnostic and therapeutic tools. There remain, however, a number of challenges that must be overcome before EVs may be used routinely in the clinic. In this review we will discuss the translational potential of EVs and the current technologies available to isolate, purify and analyse EVs and their contents.
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