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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
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Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
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Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
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Related Experiment Video

Updated: Feb 5, 2026

piggyBac Transposon System Modification of Primary Human T Cells
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Recombinant CHO Cell Pool Generation Using piggyBac Transposon System.

Sowmya Balasubramanian1,2

  • 1Laboratory of Cellular Biotechnology (LBTC), École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. sowmyab.uno@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|September 23, 2018
PubMed
Summary

This study presents a method for generating Chinese hamster ovary (CHO) cell pools for rapid recombinant protein production using the piggyBac transposon system. This approach ensures high titer and consistent product quality for efficient biomanufacturing.

Keywords:
CHO cellsOrbital shakingRecombinant proteinTransfectionTransposon system

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Area of Science:

  • Biotechnology
  • Cell line development
  • Protein expression

Background:

  • Chinese hamster ovary (CHO) cells are crucial for recombinant protein production.
  • Developing cell pools with high titer and consistent quality is essential for efficient biomanufacturing.
  • Existing methods for generating stable cell lines can be time-consuming.

Purpose of the Study:

  • To describe a novel method for generating CHO cell pools using the piggyBac transposon system.
  • To facilitate rapid production of recombinant proteins with desirable characteristics.
  • To provide a versatile platform for downstream applications like cell banking.

Main Methods:

  • Co-transfection of CHO cells with a donor plasmid (gene of interest) and a helper plasmid (transposase) using polyethyleneimine (PEI).
  • Utilizing the piggyBac transposon system for efficient and stable gene integration.
  • Employing genetic selection to isolate and generate the desired cell pool.

Main Results:

  • Successful generation of stable CHO cell pools with integrated genes of interest.
  • The developed cell pools exhibit characteristics suitable for high-titer recombinant protein production.
  • The method allows for consistent product quality across different production runs.

Conclusions:

  • The piggyBac transposon system offers an efficient strategy for generating CHO cell pools for biomanufacturing.
  • This method streamlines the process of creating high-performing cell lines for recombinant protein production.
  • The generated cell pools serve as a valuable resource for both immediate culture initiation and future cell line development.