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Subcutaneous Infection of Methicillin Resistant Staphylococcus Aureus MRSA
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The fingerprint mapping and genotyping systems application on methicillin-resistant Staphylococcus aureus

Jian Miao1, Wenxin Wang1, Wenyi Xu1

  • 1School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China.

Microbial Pathogenesis
|September 24, 2018
PubMed

Insights

This study identified 33 foodborne Staphylococci strains as methicillin-resistant Staphylococcus aureus (MRSA) using PCR. Genotyping revealed diversity among strains, highlighting multiplex-PCR

Area of Science:

  • Food Microbiology
  • Molecular Biology
  • Pathogen Detection

Background:

  • Staphylococcus aureus (S. aureus) is a common foodborne pathogen.
  • Methicillin-resistant Staphylococcus aureus (MRSA) poses significant food safety risks.
  • Understanding MRSA strain diversity is crucial for effective control.

Purpose of the Study:

  • To identify and characterize MRSA strains in food samples.
  • To evaluate different molecular typing methods for MRSA.
  • To develop a rapid detection method for MRSA.

Main Methods:

  • Multiplex-PCR was used for MRSA identification, targeting specific genes (16S rRNA, femA, mecA, orfX).
  • Three fingerprinting systems (KZ/M13, IS256, ERIC2) were employed for genotyping 33 MRSA strains.
  • Comparative analysis of typing ability, discriminatory power, and accordance ratios was performed.

Main Results:

  • All 33 Staphylococci strains were confirmed as MRSA.
  • Genotyping revealed strain diversity, with KZ/M13 and IS256 identifying 10 genotypes each, and ERIC2 identifying 8 genotypes.
  • Multiplex-PCR demonstrated rapidity and accuracy for MRSA detection.

Conclusions:

  • The developed multiplex-PCR method offers a rapid and accurate approach for MRSA detection.
  • The evaluated fingerprinting systems exhibit high sensitivity, resolution, and classification ratios for MRSA typing.
  • These molecular tools hold significant potential for application in food safety and epidemiology.
Keywords:
FingerprintingMultiplex-PCRStaphylococcus aureus

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