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Updated: Feb 4, 2026

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Published on: August 4, 2009
Recent Advances in Activity-Based Protein Profiling of Proteases
Suravi Chakrabarty1, Jan Pascal Kahler1, Merel A T van de Plassche1
1Laboratory of Chemical Biology, Department of Cellular & Molecular Medicine, KU Leuven-University of Leuven, Herestraat 49, Box 802, 3000, Louvain, Belgium.
Abstract:
The activity of proteases is tightly regulated, and dysregulation is linked to a variety of human diseases. For this reason, ABPP is a well-suited method to study protease biology and the design of protease probes has pushed the boundaries of ABPP. The development of highly selective protease probes is still a challenging task. After an introduction, the first section of this chapter discusses several strategies to enable detection of a single active protease species. These range from the usage of non-natural amino acids, combination of probes with antibodies, and engineering of the target proteases. A next section describes the different types of detection tags that facilitate the read-out possibilities including various types of imaging methods and mass spectrometry-based target identification. The power of protease ABPP is illustrated by examples for a selected number of proteases. It is expected that some protease probes that have been evaluated in animal models of human disease will find translation into clinical application in the near future.
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