PAMAM Dendrimer Modified Reduced Graphene Oxide Postfunctionalized by Horseradish Peroxidase for Biosensing H₂O₂

Area of Science:

• Biomaterials Science
• Nanotechnology
• Analytical Chemistry

Background:

• Hydrogen peroxide (H2O2) is a key biomarker in various physiological and pathological processes.
• Developing sensitive and selective biosensors for H2O2 detection in complex matrices like serum is crucial for diagnostics.

Purpose of the Study:

• To tether horseradish peroxidase (HRP) onto reduced graphene oxide (RGO) for effective H2O2 sensing in serum.
• To explore a green synthesis route for RGO and advanced functionalization techniques.

Main Methods:

• Reduced graphene oxide (RGO) synthesized via a green reduction of graphene oxide (GO) using carrot extract.
• Covalent functionalization of RGO using electrochemical amination with amine-terminated PAMAM dendrimers.
• Post-functionalization of HRP onto the RGO surface via glutaraldehyde linkage.

Main Results:

• Successful synthesis and characterization of functionalized RGO using spectroscopic, microscopic, and electrochemical methods.
• Demonstrated application of the HRP-tethered RGO biosensor for H2O2 detection in blood serum samples.
• Verified the stability and performance of the developed biosensor.

Conclusions:

• The HRP-tethered RGO platform offers a robust and sensitive approach for H2O2 sensing in serum.
• The employed green synthesis and functionalization strategy is adaptable for other carbonaceous materials.
• This work paves the way for advanced electrochemical biosensors in clinical applications.