Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.6K
CRISPR01:59

CRISPR

57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Allele Frequencies at Recessive Disease Genes are Mainly Determined by Pleiotropic Effects in Heterozygotes.

Genetics·2026
Same author

Genetic variants affect diurnal glucose levels throughout the day.

Nature communications·2026
Same author

Buffering of gene dosage response curves for human complex traits.

Cell genomics·2026
Same author

Adaptive loss of function accelerated the evolution of ancient and modern human cognition.

bioRxiv : the preprint server for biology·2026
Same author

Regulatory network topology and the genetic architecture of gene expression.

Cell genomics·2026
Same author

Genetic architectures of brain-related traits are shaped by strong selective constraints.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jan 9, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.7K

Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing.

Eilon Sharon1, Shi-An A Chen2, Neil M Khosla2

  • 1Department of Biology, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

Cell
|September 25, 2018
PubMed
Summary

Researchers used CRISPR-Cas9 genome editing to study 16,006 natural genetic variants in yeast. They identified hundreds of variants impacting fitness, mostly in promoter regions, revealing insights into genetic architecture and natural variation.

Keywords:
CRISPRCas9QTLevolutionfitnessgenetic variationgenome editingyeast

More Related Videos

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

14.4K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.4K

Related Experiment Videos

Last Updated: Jan 9, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.7K
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
08:25

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Published on: April 8, 2017

14.4K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.4K

Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Identifying genetic variants that influence natural phenotypic variation is a key challenge in genetics.
  • Current genetic mapping techniques possess limited resolution for precise variant identification.

Purpose of the Study:

  • To develop a high-throughput genome editing method for assessing the fitness consequences of numerous natural genetic variants.
  • To investigate the genetic architecture of fitness variation at single-base resolution in yeast.

Main Methods:

  • A CRISPR-Cas9-based high-throughput genome editing approach was employed to introduce thousands of specific genetic variants.
  • The fitness effects of 16,006 natural genetic variants were systematically evaluated in yeast using glucose media.

Main Results:

  • 572 genetic variants exhibited significant fitness differences, with a notable enrichment in promoter regions, especially transcription factor binding sites.
  • Only 19.2% of the identified variants directly altered amino acid sequences.
  • Clustered variants often favored alleles from the same parent, suggesting lineage-specific selection driven by multiple variants.

Conclusions:

  • The developed CRISPR-Cas9 genome editing approach offers single-base resolution for dissecting the genetic basis of fitness variation.
  • This method can be adapted for genome-wide screens to assess the impact of genetic variation on cell survival or other selectable markers.