Related Experiment Video
Updated: Feb 4, 2026

Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using
Hoang-Yen Thi Nguyen1, Gia-Buu Tran1
1Institute of Biotechnology and Food-Technology, Industrial University of Ho Chi Minh City, 12 Nguyen Van Bao Street, Go Vap, Ho Chi Minh, Vietnam.
Abstract:
Glucose isomerase is an enzyme widely used in food industry for producing high-fructose corn syrup. Many microbes, including Bacillus megaterium, have been found to be able to produce glucose isomerase. However, the number of studies of glucose isomerase production from Bacillus megaterium is limited. In this study, we establish the optimal medium components and culture conditions for Bacillus megaterium glucose isomerase production by evaluating the combined influence of multiple factors and different parameters via Plackett-Burman design and response surface methodology in Modde 5.0 software. The optimized conditions, which were experimentally confirmed as follows: D-xylose (1.116%), K2HPO4 (0.2%), MgSO4·7H2O (0.1%), yeast extract (1.161%), peptone (1%), pH 7.0, inoculum size 20% (w/v), shaking 120 rpm at 36.528°C for 48 hours, give rise to production of highest activity of glucose isomerase (0.274 ± 0.003 U/mg biomass). These results provide additional important information for future development of large-scale glucose isomerase production by Bacillus megaterium.
Related Concept Videos
Response Surface Methodology
The process of RSM involves several key steps:
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Microbial Fermentation
Glucose Homeostasis: Regulation of Blood Glucose
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Responses to Drought and Flooding

