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ESIPT-based fluorescence probe for the rapid detection of peroxynitrite 'AND' biological thiols
Luling Wu1, Hai-Hao Han, Liyuan Liu
1Department of Chemistry, University of Bath, Bath, BA2 7AY, UK. t.d.james@bath.ac.uk s.d.bull@bath.ac.uk.
Chemical Communications (Cambridge, England)
|September 25, 2018
Abstract:
An ESIPT-based 'AND' logic fluorescence probe (GSH-ABAH) was developed for the simultaneous detection of ONOO- and biological thiols. GSH-ABAH was shown to have good cell permeability and with the addition of just SIN-1 (ONOO- donor) or GSH, no fluorescence response was observed in live cells. However, in the presence of both analytes GSH-ABAH could be used to image exogenous ONOO- 'AND' GSH added to RAW264.7 cells.