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Updated: Feb 4, 2026

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci
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Simultaneous Determination of Ten Bioactive Components in Raw and Processed Radix Dipsaci by UPLC-Q-TOF-MS
Yi Tao1, Surun Huang2, Weidong Li2
1College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
Abstract:
Radix Dipsaci is employed for treating bone fractures in traditional Chinese medicine. In the current, a quantitative approach was established for determination of ten bioactive components (loganic acid, loganin, 3,5-diCQA, 4-CQA, 3,4-diCQA, sweroside, dipsacussaponin B, dipsacoside A, asperosaponin V and asperosaponin VI) in raw and rice wine-sautéed Radix Dipsaci by using UPLC-Q-TOF-MS based approach. The sample handling procedure was optimized. Chromatographic separation was operated on a Thermo Syncronis AQ-C18 UPLC column with mobile phase consisting of 0.01% aqueous formic acid and acetonitrile. The method was validated in terms of linearity, precision and recovery tests. All calibration curves displayed good linearity (R2 > 0.9991). The limit of detection and limit of quantification of these components were from 0.007 to 0.032 μg/mL and from 0.020 to 0.095 μg/mL, respectively. The average recoveries of these components were from 96.7 to 102.7% with relative standard deviation values from 0.13 to 3.81% for Radix Dipsaci. The developed approach can be employed for quality control of raw and wine-sautéed Radix Dipsaci.
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