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Related Experiment Video

Updated: Feb 4, 2026

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A Photoactivatable BODIPY Probe for Localization-Based Super-Resolution Cellular Imaging.

Chamari S Wijesooriya1, Julie A Peterson1, Pradeep Shrestha1

  • 1Department of Chemistry, Iowa State University, Ames, IA, 50011, USA.

Angewandte Chemie (International Ed. in English)
|September 25, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a new photoactivatable boron-dipyrromethene (BODIPY) probe for super-resolution microscopy. This probe uses a novel visible-light reaction for activation, enabling detailed imaging of biological structures like microtubules.

Keywords:
BODIPYfluorescenceimaging agentsphotoactivationsuper-resolution microscopy

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Area of Science:

  • Chemical Biology
  • Microscopy
  • Organic Chemistry

Background:

  • Super-resolution microscopy requires advanced fluorescent probes for high-resolution imaging.
  • Boron-dipyrromethene (BODIPY) dyes are known for their photophysical properties but often require specific activation conditions.
  • Localization-based microscopy techniques demand probes with controllable activation and photostability.

Purpose of the Study:

  • To synthesize and characterize a novel photoactivatable boron-alkylated BODIPY probe.
  • To demonstrate the probe's utility in localization-based super-resolution microscopy.
  • To showcase the probe's application in imaging biological systems, including microtubules.

Main Methods:

  • Synthesis of a boron-alkylated BODIPY derivative.
  • Photoactivation via a boron-photodealkylation reaction using a visible laser.
  • Conjugation of the probe to paclitaxel for targeted imaging.
  • Super-resolution microscopy (e.g., STORM, PALM) for in vitro and in vivo imaging.

Main Results:

  • Successful synthesis of a photoactivatable BODIPY probe.
  • Demonstration of probe activation using a single visible laser without additives.
  • Targeted labeling and super-resolution imaging of microtubules in vitro and in vivo.
  • First report of visible-light-activated BODIPY for single-molecule localization microscopy.

Conclusions:

  • The developed photoactivatable BODIPY probe offers a versatile and efficient tool for super-resolution microscopy.
  • The novel boron-photodealkylation reaction simplifies probe activation, broadening its applicability.
  • This probe enables advanced imaging of cellular structures and processes with high resolution.