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Taxonomy and phylogeny of Macrolepiota: two new species from Brazil
Eduardo Fazolino Perez1, Sandy C Suaza Blandón2, Genivaldo Alves-Silva1
1a Postgraduate Program in Botany, Institute of Biosciences , Universidade Federal do Rio Grande do Sul , Av. Bento Goncalves, 9500, Building 43433, Postal Code 91501-970 , Porto Alegre , Brazil.
Abstract:
Macrolepiota is a poorly known genus in the Neotropics. In order to increase knowledge about this group, we collected specimens from the Atlantic Forest in southern and northeastern Brazil. Macrolepiota cyanolamellata and M. sabulosa from subtropical and tropical regions, respectively, are proposed as new species. We performed molecular phylogenetic analyses of the nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS) and the combined data set ITS + nuclear large subunit rDNA (28S) + RNA polymerase II second largest (RPB2), as well as morphological analyses. Two lineages with unique morphotypes were found. The species proposed were strongly supported as the sister lineage closely related to M. clelandii and M. subcitrophylla. Detailed descriptions and illustrations of their macro- and microscopic characters are provided.
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