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Updated: Feb 4, 2026
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An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
Published on: February 9, 2017
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Ex vivo microangioCT: Advances in microvascular imaging
Ruslan Hlushchuk1, David Haberthür1, Valentin Djonov1
1Institute of Anatomy, University of Bern, Bern, Switzerland.
Vascular Pharmacology
|September 25, 2018
Summary
Microvascular imaging using microangioCT with μAngiofil offers non-destructive 3D visualization of capillaries. This technique aids in assessing pro- and anti-angiogenic compounds for cardiovascular and cancer disease treatments.
Area of Science:
- Biomedical Engineering
- Medical Imaging
- Vascular Biology
Background:
- Therapeutic angiogenesis modulation is crucial for cardiovascular and cancer diseases.
- Current preclinical assessments lack detailed vascular visualization, hindering drug development.
- Existing imaging methods fail to provide non-destructive, capillary-level 3D vascular data compatible with histology.
Purpose of the Study:
- To review the microangioCT approach with μAngiofil for enhanced microvascular imaging.
- To highlight its capability for non-destructive, high-resolution 3D vascular visualization and analysis.
- To assess its utility in preclinical studies of angiogenesis.
Main Methods:
- Microcomputed tomography (microangioCT) combined with a polymer-based contrast agent (μAngiofil).
- Time-efficient, non-destructive 3D imaging of organs and their vasculature.
- 3D-skeletonization and quantitative analysis of microvascular networks.
Main Results:
- MicroangioCT with μAngiofil enables detailed, non-destructive 3D visualization of vasculature down to the capillary level.
- The technique allows for 3D-skeletonization and quantitative analysis of microvascular architecture.
- It facilitates correlative microscopy by providing unprecedented microvascular imaging detail.
Conclusions:
- MicroangioCT with μAngiofil is a valuable tool for ex vivo microvascular research.
- It offers a simple, straightforward method for studying vascular alterations in various organs.
- This approach significantly advances preclinical assessment of angiogenesis-modulating therapies.
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