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Updated: Feb 4, 2026

Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs
Published on: March 31, 2023
Fine structural changes in the mandibular gland of the male spider crab, Libinia emarginata (L). Following eyestalk
1Department of Biology, University of South Florida, Tampa, Florida 33620 and The Marine Biological Laboratory, Woods Hole, Massachusetts 02543.
Abstract:
Mandibular glands from destalked male spider crabs were fixed in glutaraldehyde-paraformaldehyde, post-fixed in 1% osmium in phosphate buffer, dehydrated in acetone and embedded in Spurr's low-viscosity medium ('69). The glands hypertrophy and several changes occur in the subcellular cytoarchitecture following eyestalk ablation. These include loss of most of the smooth endoplasmic reticulum or its appearance as lamallae, appearance of numerous polysomes and a change in mitochondrial structure. Large vesicles with ribosomes spaced at intervals along their membranes are apparently formed by blebbing of the outer nuclear membrane and appear throughout the cytoplasm. Banded structures consisting of alternate granular and flocculent materials are found within the nucleus.
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