Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequences capable of restoring poly(A) site function define two distinct downstream elements.

M A McDevitt, R P Hart, W W Wong

    The EMBO Journal
    |November 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Patient-reported outcomes version of the common terminology criteria for adverse events and quality-of-life linear analogue self-assessment in breast cancer patients receiving radiation therapy: single-institution prospective registry.

    Journal of patient-reported outcomes·2022
    Same author

    Cardiovascular disease-related mortality and factors associated with cardiovascular events in the TREAT Asia HIV Observational Database (TAHOD).

    HIV medicine·2019
    Same author

    <i>Intellicount</i>: High-Throughput Quantification of Fluorescent Synaptic Protein Puncta by Machine Learning.

    eNeuro·2017
    Same author

    Applying recovery biomarkers to calibrate self-report measures of sodium and potassium in the hispanic community health study/study of latinos.

    Journal of human hypertension·2017
    Same author

    Applying recovery biomarkers to calibrate self-report measures of sodium and potassium in the Hispanic Community Health Study/Study of Latinos.

    Journal of human hypertension·2017
    Same author

    Smoking and projected cardiovascular risk in an HIV-positive Asian regional cohort.

    HIV medicine·2016
    Same journal

    Chromosome condensation mechanically primes the nucleus for mitosis.

    The EMBO journal·2026
    Same journal

    NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

    The EMBO journal·2026
    Same journal

    Assembly of the catalytic module and the rotor of human ATP synthase.

    The EMBO journal·2026
    Same journal

    Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC.

    The EMBO journal·2026
    Same journal

    Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.

    The EMBO journal·2026
    Same journal

    Cellular assembly and functional resilience of the mammalian RNA exosome.

    The EMBO journal·2026
    See all related articles

    Functional polyadenylation sites require specific downstream sequences for efficient cleavage. Studies show defined downstream elements, critical for primary transcript processing, are essential for gene expression regulation.

    Area of Science:

    • Molecular Biology
    • Gene Expression Regulation

    Background:

    • Polyadenylation is a crucial step in eukaryotic gene expression, involving the addition of a poly(A) tail to mRNA.
    • Recent research indicates that functional poly(A) sites involve both the conserved AAUAAA element and downstream sequences.

    Purpose of the Study:

    • To precisely locate and define critical downstream sequences essential for polyadenylation site function.
    • To investigate the role of specific downstream elements in the cleavage and polyadenylation process.

    Main Methods:

    • Utilized chemically synthesized oligonucleotides from SV40 and adenovirus E2A poly(A) sites.
    • Generated base substitution mutants within downstream sequences to assess functional impact.
    • Analyzed the effect of altering the position of downstream elements relative to the AAUAAA sequence and cleavage site.

    Related Experiment Videos

    Main Results:

    • Synthesized downstream sequences restored cleavage efficiency to a deleted SV40 poly(A) site, while inversions abolished function.
    • Specific single-base changes within downstream sequences significantly impaired poly(A) site function.
    • Optimal function was dependent on the precise positioning of downstream elements; relocation by 40 nucleotides abolished activity.
    • Differences in spacing effects and sequence composition suggest at least two distinct downstream elements exist.

    Conclusions:

    • A defined downstream sequence of limited complexity is vital for efficient primary transcript processing at the poly(A) site.
    • The spatial arrangement of these downstream elements relative to the AAUAAA motif and cleavage site is critical for function.
    • Evidence suggests the existence of at least two distinct genetic elements governing efficient poly(A) site cleavage.