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Published on: August 13, 2017
Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus
Antônio Charlys da Costa1, Adriana Luchs2, Flávio Augusto de Pádua Milagres3,4,5,6
1Institute of Tropical Medicine, University of São Paulo, São Paulo 05403-000, Brazil. charlysbr@yahoo.com.br.
Abstract:
Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes.
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