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Updated: Feb 4, 2026

Assessment of Motor Balance and Coordination in Mice using the Balance Beam

Assessment of Motor Balance and Coordination in Mice using the Balance Beam

Published on: March 10, 2011

CcpA Coordinates Growth/Damage Balance for Streptococcus pyogenes Pathogenesis.

Elyse Paluscio^1,2, Michael E Watson^1,3, Michael G Caparon⁴

¹Department of Molecular Microbiology, Washington University School of Medicine St Louis, St. Louis, MO, 63110-1093, United States.

Scientific Reports

|September 26, 2018

Summary

Pathogens balance growth and damage; Streptococcus pyogenes's Carbon Catabolite Protein A (CcpA) regulates this. Mutants showed CcpA controls virulence, impacting infection and colonization differently.

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Last Updated: Feb 4, 2026

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Published on: September 25, 2014

Area of Science:

Microbiology
Pathogenesis
Bacterial Genetics

Background:

Pathogens must balance replication and host damage for optimal fitness.
Streptococcus pyogenes utilizes Carbon Catabolite Protein A (CcpA) to regulate metabolism and virulence.
CcpA's role in coordinating growth and damage is crucial for S. pyogenes pathogenesis.

Purpose of the Study:

To investigate the role of CcpA in balancing bacterial growth and host tissue damage during infection.
To compare the virulence of wild-type S. pyogenes with CcpA-deficient and constitutively active CcpA mutants.
To elucidate how CcpA influences pathogenesis in different infection models.

Main Methods:

Utilized two distinct murine models: acute soft tissue infection and long-term asymptomatic mucosal colonization.
Generated and compared S. pyogenes mutants: one lacking CcpA and another with a constitutively DNA-binding CcpA (CcpA^T307Y).
Assessed bacterial virulence, tissue burden, and host damage in both models.

Main Results:

Both CcpA mutants exhibited altered virulence compared to wild-type.
Loss of CcpA led to reduced growth in tissues, decreased damage, and faster bacterial clearance.
Constitutive CcpA activity resulted in high bacterial loads and prolonged carriage with diminished tissue damage.

Conclusions:

CcpA is a master regulator controlling the balance between bacterial growth and host tissue damage.
The activity of CcpA actively modulates pathogen fitness and virulence strategies.
S. pyogenes virulence network topology couples carbon metabolism with growth/damage balance, influencing pathogenesis.