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Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
08:04

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Published on: March 18, 2014

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Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70

Fan Zhang1, Yanjie Zhu1, Guoxin Fan1

  • 1Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.

Oncology Letters
|September 26, 2018
PubMed

Insights

Photodynamic therapy (PDT) shows promise for osteosarcoma treatment by restoring dendritic cell function. This cancer therapy activates a photosensitizing agent with light, leading to tumor cell death and improved immune response.

Area of Science:

  • Oncology
  • Immunology
  • Biochemistry

Background:

  • Osteosarcoma is the most common primary bone cancer in children and adolescents with a poor prognosis.
  • Novel treatments are urgently needed for osteosarcoma.
  • Photodynamic therapy (PDT) is a localized cancer treatment using a photosensitizer activated by light to induce cell death.

Purpose of the Study:

  • To investigate the immune response following PDT in osteosarcoma.
  • To elucidate the molecular mechanisms underlying PDT's effect on the tumor microenvironment.

Main Methods:

  • Osteosarcoma cells were treated with PDT.
  • The effect of PDT on osteosarcoma cell necrosis was assessed.
  • Dendritic cell function was evaluated after co-culture with PDT-treated osteosarcoma cells.
  • Heat shock protein 70 (HSP70) expression was analyzed.

Main Results:

  • Osteosarcoma cell necrosis induced by PDT inhibited dendritic cell function.
  • PDT treatment of osteosarcoma cells restored dendritic cell function.
  • This restoration was mediated by the upregulation of heat shock protein 70 (HSP70).

Conclusions:

  • PDT influences the immune microenvironment in osteosarcoma.
  • PDT can modulate dendritic cell function, potentially enhancing anti-tumor immunity.
  • Upregulation of HSP70 is a key molecular event in PDT's immunomodulatory effects on osteosarcoma.
Keywords:
HSP70dendritic cellsosteosarcomaphotodynamic therapy

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