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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Multiple Puf proteins regulate the stability of ribosome biogenesis transcripts.

Anthony D Fischer1, Wendy M Olivas1

  • 1a Department of Biology , University of Missouri-St. Louis , St. Louis , MO , USA.

RNA Biology
|September 26, 2018
PubMed
Summary

Puf proteins regulate ribosome biogenesis by controlling mRNA stability. This study reveals a novel post-transcriptional mechanism impacting yeast cellular resource management and ribosome production.

Keywords:
3ʹ UTRMrna decayPuf proteinsPumiliomRNA stabilityribosome biogenesisyeast

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Area of Science:

  • Molecular Biology
  • Cellular Regulation
  • Yeast Genetics

Background:

  • Cellular resource management is critical, with ribosome biogenesis being a key regulatory area.
  • Transcriptional control of ribosome biogenesis factors via histone acetylation is established.
  • Post-transcriptional regulation offers an additional layer of control for cellular processes.

Purpose of the Study:

  • To investigate the role of Puf proteins in regulating ribosome biogenesis factor mRNA stability.
  • To identify the specific Puf proteins and mechanisms involved in this regulation.
  • To explore the functional consequences of Puf protein activity on ribosome production.

Main Methods:

  • Analysis of ribosome biogenesis factor mRNA stability in Saccharomyces cerevisiae.
  • Identification of Puf binding elements in 3' untranslated regions (UTRs).
  • Assessment of mRNA decay rates in the presence and absence of specific Puf proteins.

Main Results:

  • Several ribosome biogenesis factor mRNAs with a Puf4p element in their 3' UTR were destabilized by Puf2p, Puf4p, and Puf5p, but stabilized by Puf1p and Puf3p.
  • In the absence of all Puf proteins, these mRNAs were destabilized via a secondary mechanism involving the same 3' UTR element.
  • Overexpression of Puf4p led to delayed ribosomal RNA processing and altered ribosomal subunit trafficking.

Conclusions:

  • Puf proteins play a novel role in regulating yeast ribosome biogenesis through post-transcriptional control of mRNA stability.
  • This mechanism provides a new understanding of how cells manage resources for protein synthesis.
  • Puf protein activity directly impacts key steps in ribosome production and subunit assembly.