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Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Gas Thermometers and the Kelvin Scale01:22

Gas Thermometers and the Kelvin Scale

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The definition of temperature in terms of molecular motion suggests that there should be a lowest possible temperature, where the average kinetic energy of molecules is zero (or the minimum allowed by quantum mechanics). Experiments confirm the existence of such a temperature, called absolute zero. An absolute temperature scale is one whose zero point is absolute zero. Such scales are convenient in science because several physical quantities, such as the volume of an ideal gas, are directly...
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States of Water01:23

States of Water

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Water exists in any one of the three classical states: solid (ice), liquid (water), and gas (steam or water vapor). The state of water depends on i) the intermolecular forces that draw molecules together and ii) the kinetic energy that leads to movements that pull them apart.
Water freezes when the intermolecular forces are greater than the kinetic energy. Unlike most other substances, water is less dense in its solid state than in its liquid state. This is because each water molecule can form...
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Intermolecular Forces03:13

Intermolecular Forces

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Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
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Electromotive Force02:36

Electromotive Force

30.2K
Electricity is generated by either electrons or ions flowing through a solution or a conducting medium. This flow of electrons or specifically electrical charge is defined as an electric current. When electrons move through a wire, they generate an electric current. It can be recalled  that in a redox reaction, electrons are lost and gained. In the spontaneous redox reaction of zinc  with copper, when zinc is immersed in a copper ion solution, a transfer of electrons from one substance to...
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Intermolecular vs Intramolecular Forces03:00

Intermolecular vs Intramolecular Forces

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Intermolecular forces (IMF) are electrostatic attractions arising from charge-charge interactions between molecules. The strength of the intermolecular force is influenced by the distance of separation between molecules. The forces significantly affect the interactions in solids and liquids, where the molecules are close together. In gases, IMFs become important only under high-pressure conditions (due to the proximity of gas molecules). Intermolecular forces dictate the physical properties of...
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Updated: Feb 4, 2026

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy
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Water desorption in Kelvin-probe force microscopy: a generic model.

P Mesquida1,2, D Kohl1, S Bansode3

  • 1Automation and Control Institute (ACIN), TU Wien, Gusshausstrasse 27-29, A-1040 Vienna, Austria.

Nanotechnology
|September 26, 2018
PubMed
Summary
This summary is machine-generated.

Water desorption from nanoscale objects like nanoparticles and proteins significantly alters their measured electrostatic potential using Kelvin-probe force microscopy (KFM). This study provides a model and methods to prevent misinterpretation in atomic force microscopy (AFM) analyses.

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Area of Science:

  • Surface science
  • Nanotechnology
  • Biophysics

Background:

  • Characterizing nanoscale objects like nanoparticles and biological fibrils often requires deposition from aqueous suspension onto solid supports.
  • Atomic force microscopy (AFM) and Kelvin-probe force microscopy (KFM) are key techniques for analyzing these nanostructures.
  • Understanding surface properties, including electrostatic potential, is crucial for accurate characterization.

Purpose of the Study:

  • To investigate the impact of water desorption on the electrostatic potential of nanoscale objects measured by KFM.
  • To develop a model explaining the observed changes in electrostatic potential.
  • To propose strategies for avoiding misinterpretation in electrical AFM-based experiments.

Main Methods:

  • Utilized functionalized nanoparticles and collagen fibrils as model systems.
  • Employed Kelvin-probe force microscopy (KFM) to measure electrostatic potential.
  • Developed a simple, analytical model based on tip-sample capacitance.

Main Results:

  • Demonstrated that water desorption predictably affects the electrostatic potential of nanoparticles and collagen fibrils.
  • The observed effect is explained by a model considering the capacitance of the tip-sample system with partial dielectric filling.
  • Identified practical measures to mitigate false interpretations.

Conclusions:

  • Water desorption is a critical factor influencing electrostatic potential measurements of nanostructures using KFM.
  • The findings have significant implications for the reliable application of AFM and KFM in nanotechnology and biological sciences.
  • Accurate interpretation of electrical AFM data requires accounting for sample preparation-induced changes, such as water desorption.