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Updated: Feb 4, 2026
Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
Published on: April 7, 2013
Essential role of NbNOG1 in ribosomal RNA processing
Jiangbo Guo1,2,3, Shaojie Han2, Jinping Zhao2
1School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou 014010, China.
Abstract:
Nucleolar GTP-binding protein 1 (NOG1) is a highly conserved GTPase first reported in Trypanosoma as required for ribosome biogenesis. We characterized NbNOG1, a Nicotiana benthamiana NOG1 ortholog sharing more than 45% amino acid identity with Trypanosoma, yeast, and human NOG1. N. benthamiana plants silenced for NbNOG1 were stunted and produced sterile flowers. NbNOG1 is functionally interchangeable with yeast NOG1 (ScNOG1), rescuing yeast lethality caused by loss of ScNOG1. Finally, NbNOG1 silencing caused over-accumulation of pre-rRNA processing intermediates, and concomitant loss of mature rRNAs. Collectively, these data support a role for NbNOG1 in ribosomal RNA processing.
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