Related Experiment Video
Updated: Feb 4, 2026

In Situ Measurement of Vacuum Window Birefringence using 25Mg+ Fluorescence
Published on: June 13, 2020
Free Pentosidine Assessment Based on Fluorescence Measurements in Spent Dialysate
Sigrid Kalle1, Risto Tanner2, Merike Luman2,3
1Department of Health Technologies, Tallinn University of Technology, Tallinn, Estoniasigridkalle@gmail.com.
Abstract:
The aim of this study was to primarily explore the relationship between free pentosidine and the fluorescence properties of spent dialysate, and also to develop a model to assess the levels of free pentosidine in spent dialysate based on the fluorescence measurements. First, 40 patients (20 females and 20 males) were examined during 40 dialysis sessions. High-pressure liquid chromatography (HPLC) was used to measure the free pentosidine concentrations from the spent dialysate. The full fluorescence spectra of the spent dialysates were recorded and single- and multi-wavelength (MW) models were developed. The average free pentosidine concentrations in the spent dialysate measured by HPLC at the start and end of the dialysis session were (mean ± SD) 4.25 ± 3.11 and 0.94 ± 0.69 μg/L respectively. The removal ratios (RRs) between RR_lab and RR_MW were statistically similar (p > 0.2). The concentration of free pentosidine and the RR can therefore be estimated from the spent dialysate when utilising fluorescence measurements.
More Related Videos
09:31Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
Published on: April 15, 2017
08:49Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Published on: March 14, 2021
Related Concept Videos
Measurement: Standard Units
Measurement: Derived Units
Measures of Central Tendency
Measuring Reaction Rates
Uncertainty in Measurement: Significant Figures
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...