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Related Experiment Videos

Human cytomegalovirus replicates in primary human bone marrow cells.

H Reiser, J Kühn, H W Doerr

    The Journal of General Virology
    |December 1, 1986
    PubMed
    Summary

    Human cytomegalovirus (HCMV) replicates in human bone marrow cells, including stem cells, but with a delayed cycle. This restricted replication may explain HCMV persistence in bone marrow.

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    Area of Science:

    • Virology
    • Immunology
    • Hematology

    Background:

    • Human cytomegalovirus (HCMV) is a common pathogen with complex pathogenesis.
    • Understanding HCMV replication in hematopoietic cells is crucial for explaining viral persistence and latency.

    Purpose of the Study:

    • To investigate the replication of HCMV in primary human bone marrow cells (BMC).
    • To elucidate the role of BMC in HCMV pathogenesis and viral persistence.

    Main Methods:

    • Culture of primary human bone marrow cells (BMC).
    • Infection of BMC with HCMV AD169.
    • Analysis of viral protein and DNA synthesis.
    • Cell population enrichment and depletion experiments.
    • Indirect double immunofluorescence assays.

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    Main Results:

    • BMC are susceptible to HCMV infection.
    • HCMV replication in BMC is delayed compared to human embryonic lung cells.
    • Productive HCMV infection is restricted to a subset (≤21%) of BMC.
    • Both bone marrow fibroblasts and a subset of bone marrow stem cells support productive HCMV infection.
    • HCMV exhibits short-term persistence (over 3 months) in BMC cultures.

    Conclusions:

    • HCMV replicates in primary human bone marrow cells, including stem cells.
    • Delayed replication and restricted tropism in BMC contribute to HCMV persistence.
    • These findings offer insights into HCMV pathogenesis, including its presence in peripheral blood leukocytes.