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Complement 7 Is Up-Regulated in Human Early Diabetic Kidney Disease
Monica Sircar1, Ivy A Rosales1, Martin K Selig1
1Division of Nephrology, Massachusetts General Hospital and Partners Health Care, Harvard Medical School, Boston, Massachusetts.
The American Journal of Pathology
|September 27, 2018
Summary
Researchers identified complement 7 (C7) as a potential biomarker for early diabetic nephropathy (EDN). Elevated C7 levels in kidney tissue and blood suggest its role in EDN detection and treatment.
Area of Science:
- Nephrology
- Genomics
- Immunology
Background:
- Diabetic nephropathy (DN) progresses silently before overt kidney disease appears.
- Current methods lack the ability to detect early diabetic nephropathy (EDN).
- Identifying molecular markers for EDN is crucial for timely intervention.
Purpose of the Study:
- To identify novel candidate genes associated with early diabetic nephropathy (EDN) through unbiased gene-expression analysis.
- To investigate the role of complement 7 (C7) as a potential biomarker for EDN.
Main Methods:
- Unbiased gene-expression analysis of postmortem human kidney tissues from EDN patients and controls.
- Differential transcriptome analysis to identify significantly altered pathways and genes.
- Validation of C7 expression using real-time PCR and immunohistochemistry in kidney tissues.
- Measurement of serum C7 protein levels in EDN and control cohorts.
Main Results:
- The complement pathway was significantly altered in EDN kidneys.
- Complement 7 (C7) expression was significantly elevated in EDN kidney tissues compared to controls.
- Elevated C7 protein levels were observed in proximal tubules of EDN kidneys.
- Serum C7 protein levels were significantly higher in EDN patients than in controls, confirmed in an independent cohort.
Conclusions:
- Complement 7 (C7) is significantly associated with early diabetic nephropathy (EDN).
- C7 shows potential as a molecular target for the detection of EDN.
- C7 may also serve as a therapeutic target for managing EDN.
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