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A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
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Exploring genetic variability in Prosopis cineraria using two gene targeted CAAT box-derived polymorphism (CBDP) and
Jatan K Shekhawat1, Manoj K Rai1,2, N S Shekhawat1
1Biotechnology Unit, Department of Botany (UGC-Centre of Advanced Study), Jai Narain Vyas University, Jodhpur, Rajasthan, 342001, India.
Molecular Biology Reports
|September 27, 2018
Summary
Genetic diversity in Prosopis cineraria was assessed using CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) markers. High genetic differentiation and low gene flow were observed across Indian Thar Desert populations.
Area of Science:
- Plant genetics
- Molecular ecology
- Conservation biology
Background:
- Prosopis cineraria is vital for agroforestry and known for abiotic stress tolerance.
- Understanding its genetic diversity is crucial for conservation and sustainable utilization.
- The Indian Thar Desert harbors important wild populations of this species.
Purpose of the Study:
- To evaluate the genetic diversity and relatedness of Prosopis cineraria populations.
- To utilize gene-targeted molecular markers for genetic analysis.
- To inform conservation strategies for P. cineraria in the Thar Desert.
Main Methods:
- Employed two gene-targeted molecular marker systems: CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) polymorphism.
- Analyzed genetic variation in 49 individuals from ten wild populations across the Indian Thar Desert.
- Utilized 17 primers (10 CBDP, 7 SCoT) to generate and analyze polymorphic DNA bands.
Main Results:
- A total of 204 bands were generated, with 76.8% being polymorphic.
- High levels of genetic differentiation (GST = 0.341) and a high fixation index (FST = 0.415) were observed.
- Molecular Variance Analysis (AMOVA) indicated greater variation within populations (77%) than among populations (23%).
Conclusions:
- CBDP and SCoT markers effectively revealed high genetic differentiation and low gene flow in Prosopis cineraria populations.
- Genetic diversity varied among populations, with NGBAR and CHR showing higher diversity than SIK.
- Clustering analysis confirmed distinct genetic relationships among genotypes from different regions of the Indian Thar Desert.
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