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Updated: Feb 4, 2026

Boldness, Aggression, and Shoaling Assays for Zebrafish Behavioral Syndromes
Published on: August 29, 2016
Acute fluoxetine differently affects aggressive display in zebrafish phenotypes
Hellen P Barbosa1, Monica G Lima-Maximino1,2, Caio Maximino2,3,4
1Laboratório de Neurociências e Comportamento "Frederico Guilherme Graeff", Departamento de Morfologia e Ciências Fisiológicas, Campus VIII/Marabá, Universidade do Estado do Pará, Marabá, Pará, Brazil.
Abstract:
Zebrafish have been introduced as a model organism in behavioral neuroscience and biological psychiatry, increasing the breadth of findings using fish to study the neurobiology of aggression. Phenotypic differences between leopard and longfin zebrafish were exploited in order to elucidate the role of phasic serotonin in aggressive displays on this species. The present study, revealed differences in aggressive display between leopard and longfin zebrafish, and a discrepant effect of acute fluoxetine in both populations. In mirror-induced aggression, leopard animals showed higher display latencies than longfin, as well as lower display duration and frequency (Experiment 1). Moreover, 2.5 mg/kg fluoxetine decreased the duration and frequency of display in longfin, but not leopard; and 5 mg/kg fluoxetine increased display frequency in leopard, but not longfin (Experiment 2). It is suggested that zebrafish from the longfin phenotype show more aggressive motivation and readiness in the mirror-induced aggression test than leopard, and that acute fluoxetine increases aggression in leopard and decreased it in longfin zebrafish.
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